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brdu quantification  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology brdu quantification
    ( A ) Representative images of PLA on HDF shows STING-Lamin A interaction (foci) in control and progerin (PG)-expressing cells. DAPI is shown labeling genomic DNA. ( B ) Immunofluorescence analysis of <t>BrdU</t> incorporation in HDF treated with vehicle or doxycycline (Doxy) for 4 or 8 days to induce GFP-progerin, with or without STING inhibitor H151. ( C ) BrdU incorporation in HDF and progerin-expressing HDF transfected with STING siRNA (siSTING) or non-targeting control (siSCR). ( D ) Immunoblot analysis of STING, GFP-progerin, and phosphorylated RPA <t>(</t> <t>S33</t> p-RPA) in HDF control and progerin-expressing HDF in which STING is abrogated (H151 or siSTING). ( E ) IF images showing S33 p-RPA foci (red) in cells treated with Doxy ± H151 for 4 or 8 days; DAPI (blue) labels nuclei and white arrows indicate cytosolic foci. Scale bars, 20 µm ( F ) Quantification of cells with ≥3 nuclear S33 p-RPA foci per nucleus from (E). ( G ) Quantification of cells with nuclear S33 p-RPA foci in siSTING-or control siSCR-transfected cells treated with Doxy for 8 days. ( H ) Percentage of cells showing with ≥3 cytosolic S33 p-RPA foci from (E). ( I ) Cytosolic S33 p-RPA foci quantification in siSTING versus siSCR-transfected cells.
    Brdu Quantification, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1197 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/brdu+quantification/BrdU+Antibody/bio_rxiv__64898__2026__03__28__714577-207-18-21
    Average 96 stars, based on 1197 article reviews
    brdu quantification - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "STING causes replication stress and nascent DNA degradation via SAMHD1"

    Article Title: STING causes replication stress and nascent DNA degradation via SAMHD1

    Journal: bioRxiv

    doi: 10.64898/2026.03.28.714577

    ( A ) Representative images of PLA on HDF shows STING-Lamin A interaction (foci) in control and progerin (PG)-expressing cells. DAPI is shown labeling genomic DNA. ( B ) Immunofluorescence analysis of BrdU incorporation in HDF treated with vehicle or doxycycline (Doxy) for 4 or 8 days to induce GFP-progerin, with or without STING inhibitor H151. ( C ) BrdU incorporation in HDF and progerin-expressing HDF transfected with STING siRNA (siSTING) or non-targeting control (siSCR). ( D ) Immunoblot analysis of STING, GFP-progerin, and phosphorylated RPA ( S33 p-RPA) in HDF control and progerin-expressing HDF in which STING is abrogated (H151 or siSTING). ( E ) IF images showing S33 p-RPA foci (red) in cells treated with Doxy ± H151 for 4 or 8 days; DAPI (blue) labels nuclei and white arrows indicate cytosolic foci. Scale bars, 20 µm ( F ) Quantification of cells with ≥3 nuclear S33 p-RPA foci per nucleus from (E). ( G ) Quantification of cells with nuclear S33 p-RPA foci in siSTING-or control siSCR-transfected cells treated with Doxy for 8 days. ( H ) Percentage of cells showing with ≥3 cytosolic S33 p-RPA foci from (E). ( I ) Cytosolic S33 p-RPA foci quantification in siSTING versus siSCR-transfected cells.
    Figure Legend Snippet: ( A ) Representative images of PLA on HDF shows STING-Lamin A interaction (foci) in control and progerin (PG)-expressing cells. DAPI is shown labeling genomic DNA. ( B ) Immunofluorescence analysis of BrdU incorporation in HDF treated with vehicle or doxycycline (Doxy) for 4 or 8 days to induce GFP-progerin, with or without STING inhibitor H151. ( C ) BrdU incorporation in HDF and progerin-expressing HDF transfected with STING siRNA (siSTING) or non-targeting control (siSCR). ( D ) Immunoblot analysis of STING, GFP-progerin, and phosphorylated RPA ( S33 p-RPA) in HDF control and progerin-expressing HDF in which STING is abrogated (H151 or siSTING). ( E ) IF images showing S33 p-RPA foci (red) in cells treated with Doxy ± H151 for 4 or 8 days; DAPI (blue) labels nuclei and white arrows indicate cytosolic foci. Scale bars, 20 µm ( F ) Quantification of cells with ≥3 nuclear S33 p-RPA foci per nucleus from (E). ( G ) Quantification of cells with nuclear S33 p-RPA foci in siSTING-or control siSCR-transfected cells treated with Doxy for 8 days. ( H ) Percentage of cells showing with ≥3 cytosolic S33 p-RPA foci from (E). ( I ) Cytosolic S33 p-RPA foci quantification in siSTING versus siSCR-transfected cells.

    Techniques Used: Control, Expressing, Labeling, Immunofluorescence, BrdU Incorporation Assay, Transfection, Western Blot

    Related Articles

    Immunofluorescence:

    Article Title: STING causes replication stress and nascent DNA degradation via SAMHD1
    Article Snippet: Western blot images were acquired using SYNGENE PXi instrument. .. Immunofluorescence was performed to analyze STING localization and activation (STING 1:500-Cell Signaling- 13647, S366 pSTING 1:1200- Cell Signaling-50907), BrdU quantification (1:200- Santa Cruz -sc20045), levels of S33 p-RPA (1:1000- Bethyl -PLA0070), γH2AX (1:200 Cell Signalling-2577) and 53BP1 (1:1000- Santa Cruz- sc517281). ..

    Activation Assay:

    Article Title: STING causes replication stress and nascent DNA degradation via SAMHD1
    Article Snippet: Western blot images were acquired using SYNGENE PXi instrument. .. Immunofluorescence was performed to analyze STING localization and activation (STING 1:500-Cell Signaling- 13647, S366 pSTING 1:1200- Cell Signaling-50907), BrdU quantification (1:200- Santa Cruz -sc20045), levels of S33 p-RPA (1:1000- Bethyl -PLA0070), γH2AX (1:200 Cell Signalling-2577) and 53BP1 (1:1000- Santa Cruz- sc517281). ..



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    brdu quantification  (Santa Cruz Biotechnology)
    96
    Santa Cruz Biotechnology brdu quantification
    ( A ) Representative images of PLA on HDF shows STING-Lamin A interaction (foci) in control and progerin (PG)-expressing cells. DAPI is shown labeling genomic DNA. ( B ) Immunofluorescence analysis of <t>BrdU</t> incorporation in HDF treated with vehicle or doxycycline (Doxy) for 4 or 8 days to induce GFP-progerin, with or without STING inhibitor H151. ( C ) BrdU incorporation in HDF and progerin-expressing HDF transfected with STING siRNA (siSTING) or non-targeting control (siSCR). ( D ) Immunoblot analysis of STING, GFP-progerin, and phosphorylated RPA <t>(</t> <t>S33</t> p-RPA) in HDF control and progerin-expressing HDF in which STING is abrogated (H151 or siSTING). ( E ) IF images showing S33 p-RPA foci (red) in cells treated with Doxy ± H151 for 4 or 8 days; DAPI (blue) labels nuclei and white arrows indicate cytosolic foci. Scale bars, 20 µm ( F ) Quantification of cells with ≥3 nuclear S33 p-RPA foci per nucleus from (E). ( G ) Quantification of cells with nuclear S33 p-RPA foci in siSTING-or control siSCR-transfected cells treated with Doxy for 8 days. ( H ) Percentage of cells showing with ≥3 cytosolic S33 p-RPA foci from (E). ( I ) Cytosolic S33 p-RPA foci quantification in siSTING versus siSCR-transfected cells.
    Brdu Quantification, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 1 article reviews
    brdu quantification - by Bioz Stars, 2026-09
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    quantification of brdu  (MetaMorph Inc)
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    ( A ) Representative images of PLA on HDF shows STING-Lamin A interaction (foci) in control and progerin (PG)-expressing cells. DAPI is shown labeling genomic DNA. ( B ) Immunofluorescence analysis of <t>BrdU</t> incorporation in HDF treated with vehicle or doxycycline (Doxy) for 4 or 8 days to induce GFP-progerin, with or without STING inhibitor H151. ( C ) BrdU incorporation in HDF and progerin-expressing HDF transfected with STING siRNA (siSTING) or non-targeting control (siSCR). ( D ) Immunoblot analysis of STING, GFP-progerin, and phosphorylated RPA <t>(</t> <t>S33</t> p-RPA) in HDF control and progerin-expressing HDF in which STING is abrogated (H151 or siSTING). ( E ) IF images showing S33 p-RPA foci (red) in cells treated with Doxy ± H151 for 4 or 8 days; DAPI (blue) labels nuclei and white arrows indicate cytosolic foci. Scale bars, 20 µm ( F ) Quantification of cells with ≥3 nuclear S33 p-RPA foci per nucleus from (E). ( G ) Quantification of cells with nuclear S33 p-RPA foci in siSTING-or control siSCR-transfected cells treated with Doxy for 8 days. ( H ) Percentage of cells showing with ≥3 cytosolic S33 p-RPA foci from (E). ( I ) Cytosolic S33 p-RPA foci quantification in siSTING versus siSCR-transfected cells.
    Quantification Of Brdu, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/brdu+quantification/quantification+of+brdu/pmc03077085__NIHMS260798___supplement___1-58-3-0
    Average 90 stars, based on 1 article reviews
    quantification of brdu - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A ) Representative images of PLA on HDF shows STING-Lamin A interaction (foci) in control and progerin (PG)-expressing cells. DAPI is shown labeling genomic DNA. ( B ) Immunofluorescence analysis of BrdU incorporation in HDF treated with vehicle or doxycycline (Doxy) for 4 or 8 days to induce GFP-progerin, with or without STING inhibitor H151. ( C ) BrdU incorporation in HDF and progerin-expressing HDF transfected with STING siRNA (siSTING) or non-targeting control (siSCR). ( D ) Immunoblot analysis of STING, GFP-progerin, and phosphorylated RPA ( S33 p-RPA) in HDF control and progerin-expressing HDF in which STING is abrogated (H151 or siSTING). ( E ) IF images showing S33 p-RPA foci (red) in cells treated with Doxy ± H151 for 4 or 8 days; DAPI (blue) labels nuclei and white arrows indicate cytosolic foci. Scale bars, 20 µm ( F ) Quantification of cells with ≥3 nuclear S33 p-RPA foci per nucleus from (E). ( G ) Quantification of cells with nuclear S33 p-RPA foci in siSTING-or control siSCR-transfected cells treated with Doxy for 8 days. ( H ) Percentage of cells showing with ≥3 cytosolic S33 p-RPA foci from (E). ( I ) Cytosolic S33 p-RPA foci quantification in siSTING versus siSCR-transfected cells.

    Journal: bioRxiv

    Article Title: STING causes replication stress and nascent DNA degradation via SAMHD1

    doi: 10.64898/2026.03.28.714577

    Figure Lengend Snippet: ( A ) Representative images of PLA on HDF shows STING-Lamin A interaction (foci) in control and progerin (PG)-expressing cells. DAPI is shown labeling genomic DNA. ( B ) Immunofluorescence analysis of BrdU incorporation in HDF treated with vehicle or doxycycline (Doxy) for 4 or 8 days to induce GFP-progerin, with or without STING inhibitor H151. ( C ) BrdU incorporation in HDF and progerin-expressing HDF transfected with STING siRNA (siSTING) or non-targeting control (siSCR). ( D ) Immunoblot analysis of STING, GFP-progerin, and phosphorylated RPA ( S33 p-RPA) in HDF control and progerin-expressing HDF in which STING is abrogated (H151 or siSTING). ( E ) IF images showing S33 p-RPA foci (red) in cells treated with Doxy ± H151 for 4 or 8 days; DAPI (blue) labels nuclei and white arrows indicate cytosolic foci. Scale bars, 20 µm ( F ) Quantification of cells with ≥3 nuclear S33 p-RPA foci per nucleus from (E). ( G ) Quantification of cells with nuclear S33 p-RPA foci in siSTING-or control siSCR-transfected cells treated with Doxy for 8 days. ( H ) Percentage of cells showing with ≥3 cytosolic S33 p-RPA foci from (E). ( I ) Cytosolic S33 p-RPA foci quantification in siSTING versus siSCR-transfected cells.

    Article Snippet: Immunofluorescence was performed to analyze STING localization and activation (STING 1:500-Cell Signaling- 13647, S366 pSTING 1:1200- Cell Signaling-50907), BrdU quantification (1:200- Santa Cruz -sc20045), levels of S33 p-RPA (1:1000- Bethyl -PLA0070), γH2AX (1:200 Cell Signalling-2577) and 53BP1 (1:1000- Santa Cruz- sc517281).

    Techniques: Control, Expressing, Labeling, Immunofluorescence, BrdU Incorporation Assay, Transfection, Western Blot